Figure7 shows the obtained derivative ratio spectra of different concentrations of CAF using scaling factor=1 and =8nm. Finally another novel method for simultaneous determination of quaternary mixtures was proposed and named double divisor-ratio difference-dual wave length (DD-RD-DW). Suppose the amplitudes of the ratio spectra of the ternary mixture at the two selected wavelength (iso1 and iso2 between X and Y) are P1 and P2, respectively, then; The concentration of Z is calculated using the regression equation representing the linear correlation between the differences of ratio spectra amplitudes at the two selected wavelengths to the corresponding concentrations of drug (Z). Bethesda, MD 20894, Web Policies Results are presented for hematoma diameters of (a)5.0 and (b)8.5cm. MAH revised the manuscript and the data, collected the data, and put the theoretical background. Principles of pulse oximetry. A dual wavelength method of continuous illumination spectroscopy used 760 nm (deoxyhemoglobin peak) and 800 nm (an oxyhemoglobin-deoxyhemoglobin isosbestic point) to measure the kinetics and extent of oxyhemoglobin deoxygenation in brains during mild ischemia/hypoxia. The LibreTexts libraries arePowered by NICE CXone Expertand are supported by the Department of Education Open Textbook Pilot Project, the UC Davis Office of the Provost, the UC Davis Library, the California State University Affordable Learning Solutions Program, and Merlot. Oxyhaemoglobin and deoxyhaemoglobin have (not exclusively) isosbestic points at 586 nm and near 808 nm. HbA1c (Hemoglobin A1c): A1c Chart, Test, Levels, & Normal Range - WebMD isosbestic: ( 'sos-bes'tik ), Denoting the wavelength of light at which two related compounds have identical extinction coefficients; for example, the wavelength at which the absorption spectra of hemoglobin and oxyhemoglobin cross is their isosbestic point. New York: Oxford University Press, 2000. Different applications of isosbestic points, normalized spectra and dual wavelength as powerful tools for resolution of multicomponent mixtures with severely overlapping spectra, $${\text{P}}_{ 1} = \left[ {{\text{C}}_{\text{x}} } \right]\; + \;\left[ {{\text{C}}_{\text{Y}} } \right]\; + \;\left[ {{\text{a}}_{\text{z1}} {\text{C}}_{\text{z}} } \right]/{\text{a}}_{\text{x}}$$, $${\text{P}}_{ 2} = \;\left[ {{\text{C}}_{\text{x}} } \right]\; + \;\left[ {{\text{C}}_{\text{Y}} } \right]\; + \;\left[ {{\text{a}}_{\text{z2}} {\text{ C}}_{\text{z}} } \right]/{\text{a}}_{\text{x}}$$, $${\text{P}}_{ 1} - {\text{P}}_{ 2} = \left( {\frac{{a_{z} C_{z} }}{{a_{x} }}} \right)1 - \left( {\frac{{a_{z} C_{z} }}{{a_{x} }}} \right)2$$, $${\text{Pm}}_{ 1} = {\text{P}}_{\text{X1}} \; + \;{\text{P}}_{\text{Y1}} \quad {\text{at }}\lambda_{ 1}$$, $${\text{Pm}}_{ 2} = {\text{P}}_{\text{X2}} \; + \;{\text{P}}_{\text{Y2}} \quad {\text{at }}\lambda_{ 2}$$, $${\text{F}}_{\text{Y}} = {\text{P}}_{\text{Y1}} /{\text{P}}_{\text{Y2}}$$, $$\therefore {\text{P}}_{\text{Y1}} = {\text{F}}_{\text{Y}} {\text{P}}_{\text{Y2}}$$, $${\text{Pm}}_{ 1} = {\text{P}}_{\text{X1}} \; + \;{\text{F}}_{\text{Y}} {\text{P}}_{\text{Y2}}$$, $${\text{F}}_{\text{Y}} {\text{Pm}}_{ 2} = {\text{F}}_{\text{Y}} {\text{P}}_{\text{X2}} \; + \;{\text{F}}_{\text{Y}} {\text{P}}_{\text{Y2}}$$, $$\Delta {\text{P }}\left( {{\text{Pm}}_{ 1} \;{-}\;{\text{F}}_{\text{Y}} {\text{Pm}}_{ 2} } \right) = {\text{A}}_{\text{X1}} \; - \;{\text{F}}_{\text{Y}} {\text{A}}_{\text{X2}}$$, $${\text{Pm}} = \frac{{a_{X} C_{X} }}{{a_{Z} + a_{W} }}\; + \,\frac{{a_{Y} C_{Y} }}{{a_{Z} + a_{W} }}\; + \;{\text{constant}}$$, $${\text{P}}_{ 1} = \frac{{a_{X} C_{X} }}{{[a_{Z} + a_{W} ]1}} + \frac{{a_{Y} C_{Y} }}{{[a_{Z} + a_{W} ]1}} + {\text{constant}}$$, $${\text{P}}_{ 2} = \frac{{a_{X} C_{X} }}{{[a_{Z} + a_{W} ]2}} + \frac{{a_{Y} C_{Y} }}{{[a_{Z} + a_{W} ]2}} + {\text{constant}}$$, $$\frac{{a_{Y} C_{Y} }}{{[a_{Z}\,+\,a_{W} ]1}} = \frac{{a_{Y} C_{Y} }}{{[a_{Z}\,+\,a_{W} ]2}}$$, $${\text{P}}_{ 1} - {\text{P}}_{ 2} = \left( {\frac{{a_{X} C_{X} }}{{a_{Z + } a_{W} }}} \right)1 - \left( {\frac{{a_{X} C_{X} }}{{a_{Z + } a_{W} }}} \right)2$$, \(\left( {{\text{F}} \; = \; \left[ {{\text{P}}_{ 2 6 5} /{\text{P}}_{ 2 9 5} } \right] \; = \; 5.

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